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KMID : 1134820000290020300
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Journal of the Korean Society of Food Science and Nutrition
2000 Volume.29 No. 2 p.300 ~ p.306
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Evaluation on the Safety of γ - Irradiated Angelica gigas Nakai: Stability of Active Components and Safety in Genotoxicity Test
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Yu Young-Bub
Jo Sung-Kee
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Abstract
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In the present studies, we assessed the stability of active components and toxicological safety of irradiated Angelica gigas Nakai (Danggui). In order to confirm the stability of active components in the γ-irradiated roots of Danggui, the quantitative analysis of decursin and decursinol angelate of γ-irradiated sample was carried out by high performance liquid chromatographic (HPLC) methods using reverse phase columns and normal phase columns. From the root of Danggui, decursin and decursinol angelate were isolated by a silica gel column chromatography (toluene : ether (1 : 1), Hexane : EtOAc (15 : 1)). And then the struc- tures were confirmed in the ¹H and ¹³C-NMR analysis. The HPLC chromatograms of decursin and decursinol angelate in γ-irradiated Danggui were similar with those of non-irradiated sample. In the examination of in vitro genotoxicity of the water extract from γ-irradiated Danggui using Salmonella reversion assay (Ames test) and micronucleus test in Chinese hamster ovary (CHO) cells, mutagenicity was not exhibited in the two assays with or without metabolic activation. These results suggest that active components in the γ-irradiated Danggui should be stable and that the safety of γ-irradiated Danggui could be revealed in further tests in vivo.
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KEYWORD
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${\gamma}$-irradiated Angelica gigas Nakai, decursin, decursionol angelate, HPLC, Ames test, micronucleus test
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